Journal: Bioactive Materials

Article Title: Sulfated liposome-based artificial cell membrane glycocalyx nanodecoys for coronavirus inactivation by membrane fusion

doi: 10.1016/j.bioactmat.2023.10.021

Figure Lengend Snippet: Nanodecoy membrane fusion causes virus block. The cryo-TEM image of (A) SARS-CoV-2 or (B) SARS-CoV-2 incubated with 40S_Lip. The arrow pointing the spike protein. Scale bar: 50 nm. (C) The membrane fusion profile of the FRET lipid labeled nanodecoy 40S_Lip(FRET) incubated with no FRET lipid labeled nanodecoy 40S_Lip, SARS-CoV-2 pseudovirus, or 40S_Lip and SARS-CoV-2 pseudovirus. (D) The MD simulation result of the spike protein incubated with 40S_Lip for 0, 0.12, and 2 ns. (E) The potential of mean force curves for 40S_Lip and S protein. (F) The CD spectrum of the native spike protein or when incubated with 40S_Lip. (G) The percentage of the detected free spike protein after incubation with heparin, 40S_Lip(Large), or 40S_Lip. (H) The percentage of the detected spike protein on the SARS-CoV-2 pseudovirus after incubation with heparin, 40S_Lip(Large), or 40S_Lip.

Article Snippet: The SARS-CoV-2 spike protein S1 RBD ELISA kit was purchased from Elabscience Biotechnology Co., Ltd. (Wuhan, China).

Techniques: Membrane, Virus, Blocking Assay, Incubation, Labeling

Journal: ACS ES&T water

Article Title: Simple Affinity-Based Method for Concentrating Viruses from Wastewater Using Engineered Curli Fibers

doi: 10.1021/acsestwater.1c00208

Figure Lengend Snippet: Spin down of purified, recombinant SARS-CoV-2 spike protein from PBS using engineered curli fibers. A live-cell ELISA assay was developed to probe for the presence of spike protein in the curli-cell pellet following the spin-down protocol. The four bars on the left correspond to various negative controls, and the remaining 10 bars correspond to samples where the curli fibers were engineered to display protein or peptide domains with putative binding affinity for the SARS-CoV-2 spike protein. Higher absorbance readings correspond to increased immobilization of the antibody used to detect the presence of the recombinant spike protein in the cell pellet. Values and error bars reflect the mean ± s.e. of three independent experiments ( n = 3).

Article Snippet: The mixture was incubated end-over-end at room temperature for 5 min. Then, 1 μ L of recombinant SARS-CoV-2 spike protein subunit S1 with hFc and His tags (Abclonal RP01259) was added to reach a final concentration of 0.2 μ g/mL.

Techniques: Purification, Recombinant, Enzyme-linked Immunosorbent Assay, Binding Assay